Download MendeleyBacterial selenocysteine synthase structure revealed by single-particle cryoEM
Balasco Serrao, V.H., Minari, K., Pereira, H.M., Thiemann, O.H.(2024) Curr Res Struct Biol 7
8UZW
Selenocysteine synthase- SelA
- PDB DOI: https://doi.org/10.2210/pdb8UZW/pdb
- EM Map EMD-42845: EMDB EMDataResource
- Classification: RNA BINDING PROTEIN
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2023-11-16 Released: 2024-04-24
- Funding Organization(s): Sao Paulo Research Foundation (FAPESP), National Institutes of Health/National Center for Research Resources (NIH/NCRR), Brazilian National Council for Scientific and Technological Development (CNPq)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 2.69 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| L-seryl-tRNA(Sec) selenium transferase | 463 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: selA, fdhA, b3591, JW3564 EC: 2.9.1.1 |  | |
UniProt | |||||
Find proteins for P0A821 (Escherichia coli (strain K12)) Explore P0A821 Go to UniProtKB: P0A821 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0A821 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| LLP Query on LLP | A, B, C, D, E A, B, C, D, E, F, G, H, I, J | L-PEPTIDE LINKING | C14 H22 N3 O7 P |  | LYS |
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 2.69 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
| Task | Software Package | Version |
|---|---|---|
| RECONSTRUCTION | cryoSPARC | 4.2.1 |
| MODEL REFINEMENT | PHENIX | 1.20.1-4487 |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-04-24 Deposition Author(s): Balasco Serrao, V.H., Minari, K., Pereira, H.M., Thiemann, O.H.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Sao Paulo Research Foundation (FAPESP) | Brazil | 12/23730-1 |
| National Institutes of Health/National Center for Research Resources (NIH/NCRR) | United States | U24GM129547 |
| National Institutes of Health/National Center for Research Resources (NIH/NCRR) | United States | S10OD02509 |
| Brazilian National Council for Scientific and Technological Development (CNPq) | Brazil | 232251/2014-2 |
| Brazilian National Council for Scientific and Technological Development (CNPq) | Brazil | 140636/2013-7 |
Revision History (Full details and data files)
- Version 1.0: 2024-04-24
Type: Initial release - Version 1.1: 2024-05-01
Changes: Database references, Experimental preparation
