8SJP

[3T15] Self-assembling DNA motif with 15 base pairs between junctions and P32 symmetry

  • Classification: DNA
  • Organism(s): synthetic construct
  • Mutation(s): No 

  • Deposited: 2023-04-18 Released: 2024-04-24 
  • Deposition Author(s): Vecchioni, S., Janowski, J., Sha, R., Ohayon, Y.P.
  • Funding Organization(s): Office of Naval Research (ONR), Department of Energy (DOE, United States), National Science Foundation (NSF, United States), Human Frontier Science Program (HFSP), National Aeronautic Space Administration (NASA, United States)

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 5.22 Å
  • R-Value Free: 0.161 
  • R-Value Work: 0.093 
  • R-Value Observed: 0.096 

wwPDB Validation   3D Report Full Report


This is version 1.0 of the entry. See complete history. 


Literature

Programming Tertiary Chirality in Self-Assembling 3D DNA Crystals

Janowski, J., Vecchioni, S., Sha, R., Ohayon, Y.P., Woloszyn, K., Lu, B., Perren, L., Jonoska, N.

To be published.

Macromolecules

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Entity ID: 1
MoleculeChains LengthOrganismImage
DNA (5'-D(*CP*AP*GP*CP*TP*GP*AP*CP*CP*TP*GP*AP*CP*TP*CP*AP*CP*A)-3')18synthetic construct
Sequence Annotations
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  • Reference Sequence

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Entity ID: 2
MoleculeChains LengthOrganismImage
DNA (5'-D(P*CP*GP*AP*TP*GP*GP*AP*CP*AP*GP*GP*GP*G)-3')13synthetic construct
Sequence Annotations
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  • Reference Sequence

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Entity ID: 3
MoleculeChains LengthOrganismImage
DNA (5'-D(P*TP*GP*TP*GP*AP*GP*TP*CP*AP*CP*CP*AP*TP*CP*G)-3')15synthetic construct
Sequence Annotations
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  • Reference Sequence

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Entity ID: 4
MoleculeChains LengthOrganismImage
DNA (5'-D(*TP*GP*CP*TP*CP*CP*TP*GP*TP*GP*GP*TP*CP*AP*GP*C)-3')16synthetic construct
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 5.22 Å
  • R-Value Free: 0.161 
  • R-Value Work: 0.093 
  • R-Value Observed: 0.096 
  • Space Group: P 32
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 100.632α = 90
b = 100.632β = 90
c = 72.676γ = 120
Software Package:
Software NamePurpose
PHENIXrefinement
autoPROCdata reduction
STARANISOdata scaling
PHASERphasing

Structure Validation

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Entry History & Funding Information

Deposition Data

Funding OrganizationLocationGrant Number
Office of Naval Research (ONR)United StatesN000141912596
Department of Energy (DOE, United States)United StatesDE-SC0007991
National Science Foundation (NSF, United States)United States2106790
Human Frontier Science Program (HFSP)FranceRPG0010/2017
National Science Foundation (NSF, United States)United StatesDMR-1420073
National Aeronautic Space Administration (NASA, United States)United States2020 NASA Center Innovation Fund

Revision History  (Full details and data files)

  • Version 1.0: 2024-04-24
    Type: Initial release