6N4Q

CryoEM structure of Nav1.7 VSD2 (actived state) in complex with the gating modifier toxin ProTx2


ELECTRON MICROSCOPY
Sample
Nav1.7 VSD2 in complex with ProTx2 and an anti-Nav Fab
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification DetailsApply 3 uL, blot 2.5s. Ted Pella 595 filter paper.
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles187192
Reported Resolution (Å)3.6
Resolution MethodFSC 0.143 CUT-OFF
Other DetailsSpatial frequencies higher than 8 Angstroms were not used during refinement.
Refinement Type
Symmetry TypePOINT
Point SymmetryC2
Map-Model Fitting and Refinement
Id1
Refinement Space
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeGATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å**2)41
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)1000
Maximum Defocus (nm)2500
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification165000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
PARTICLE SELECTIONcisTEM1.0.0
IMAGE ACQUISITIONSerialEM
CTF CORRECTIONcisTEM1.0.0
INITIAL EULER ASSIGNMENTcisTEM1.0.0
FINAL EULER ASSIGNMENTcisTEM1.0.0
CLASSIFICATIONcisTEM1.0.0
RECONSTRUCTIONcisTEM1.0.0
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION