1BYX

CHIMERIC HYBRID DUPLEX R(GCAGUGGC).R(GCCA)D(CTGC) COMPRISING THE TRNA-DNA JUNCTION FORMED DURING INITIATION OF HIV-1 REVERSE TRANSCRIPTION

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	NOESY				7.0	1 atm	298
2	TOCSY				7.0	1 atm	298
3	DQF-COSY				7.0	1 atm	298
4	E H-P HETCOR				7.0	1 atm	298
5	H-C COSY				7.0	1 atm	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AMX	500
2	Bruker	AMX	600
3	Varian	UNITY	750

NMR Refinement
Method	Details	Software
distance geometry	REFINEMENT DETAILS ARE DESCRIBED IN THE JRNL CITATION ABOVE	DYANA

NMR Ensemble Information
Conformer Selection Criteria	DYANA TARGET FUNCTION
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20

Additional NMR Experimental Information
Details	THE 20 STRUCTURES ARE ENERGY-MINIMIZED WITH THE AMBER FORCE FIELD USING THE PROGRAM OPAL

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	DYANA		GUNTERT,WUTHRICH
2	structure solution	DYANA

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.