1AP8
TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY (1H | 6.5 | 303 | |||||
| 2 | 13C | 6.5 | 303 | |||||
| 3 | 15N) | 6.5 | 303 | |||||
| 4 | HCCHTOCSY | 6.5 | 303 | |||||
| 5 | CT-HNCA | 6.5 | 303 | |||||
| 6 | CT-HN(CO)CA | 6.5 | 303 | |||||
| 7 | HNCACB | 6.5 | 303 | |||||
| 8 | HN(CO)CACB | 6.5 | 303 | |||||
| 9 | HNCACO-D | 6.5 | 303 | |||||
| 10 | HNCO | 6.5 | 303 | |||||
| 11 | HN(COCA)NH | 6.5 | 303 | |||||
| 12 | HN(CA)HA | 6.5 | 303 | |||||
| 13 | HN(COCA)HA | 6.5 | 303 | |||||
| 14 | HCC (CO)NNH | 6.5 | 303 | |||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | UNITYPLUS750 | 750 |
| 2 | Varian | UNITY500 | 500 |
| 3 | Varian | UNITYPLUS500 | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| molecular dynamics | X-PLOR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | NO VIOLATION ABOVE 0.5 ANGSTROMS AND 5 DEGREE DIHEDRAL ANGLE |
| Conformers Calculated Total Number | 60 |
| Conformers Submitted Total Number | 20 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | 3.851 | BRUNGER |
| 2 | structure solution | X-PLOR | 3.851 | |
