Annotations: 5GPY

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Zinc beta-ribbon	8043056	3001238	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02002	e5gpyA1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF02002	ECOD (1.6)
A	PF08271	e5gpyA2	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: PF08271	ECOD (1.6)
B	PF18121	e5gpyB1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF18121	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF08271	TFIIB zinc-binding (TF_Zn_Ribbon)	TFIIB zinc-binding	The transcription factor TFIIB contains a zinc-binding motif near the N-terminus. This domain is involved in the interaction with RNA pol II and TFIIF and plays a crucial role in selecting the transcription initiation site. The domain adopts a zinc ...	The transcription factor TFIIB contains a zinc-binding motif near the N-terminus. This domain is involved in the interaction with RNA pol II and TFIIF and plays a crucial role in selecting the transcription initiation site. The domain adopts a zinc ribbon like structure [1].	Domain
A	PF02002	TFIIE alpha subunit (TFIIE_alpha)	TFIIE alpha subunit	-	Family
B	PF18121	TFA2 Winged helix domain 2 (TFA2_Winged_2)	TFA2 Winged helix domain 2	This is the second winged helix domain can be found in TFA2 proteins present in Saccharomyces cerevisiae. In form 2, the domain interacts directly with Rad3, a DNA helicase [1].	Domain
B	PF02186	TFIIE beta subunit core domain (TFIIE_beta)	TFIIE beta subunit core domain	General transcription factor TFIIE consists of two subunits, TFIIE alpha Pfam:PF02002 and TFIIE beta. TFIIE beta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymer ...	General transcription factor TFIIE consists of two subunits, TFIIE alpha Pfam:PF02002 and TFIIE beta. TFIIE beta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. The structure of the DNA binding core region has been solved [1] and has a winged helix fold.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	General transcription factor IIE subunit 1	general transcription initiation factor activity RNA polymerase II general transcription initiation factor activity molecular adaptor activity cation binding ion binding binding metal ion binding small molecule binding	protein-containing complex remodeling cellular biosynthetic process RNA metabolic process cellular component organization protein-DNA complex organization RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process transcription open complex formation at RNA polymerase II promoter organic substance metabolic process transcription by RNA polymerase II organic substance biosynthetic process cellular component organization or biogenesis protein-containing complex remodeling cellular biosynthetic process RNA metabolic process cellular component organization protein-DNA complex organization RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process transcription open complex formation at RNA polymerase II promoter organic substance metabolic process transcription by RNA polymerase II organic substance biosynthetic process cellular component organization or biogenesis transcription initiation at RNA polymerase II promoter protein-DNA complex remodeling DNA-templated transcription open complex formation protein-containing complex organization cellular metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process DNA-templated transcription initiation nucleic acid metabolic process organic cyclic compound metabolic process biosynthetic process organic cyclic compound biosynthetic process cellular process metabolic process macromolecule metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular anatomical structure cytoplasm cellular anatomical entity cytosol RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle nucleoplasm catalytic complex intracellular membrane-bounded organelle transferase complex, transferring phosphorus-containing groups nuclear DNA-directed RNA polymerase complex organelle lumen organelle RNA polymerase complex transcription factor TFIID complex DNA-directed RNA polymerase complex intracellular protein-containing complex nuclear lumen transcription factor TFIIE complex
B	Transcription initiation factor IIE subunit beta	DNA binding binding organic cyclic compound binding nucleic acid binding general transcription initiation factor activity RNA polymerase II general transcription initiation factor activity RNA binding	cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process transcription by RNA polymerase II organic substance metabolic process organic substance biosynthetic process transcription initiation at RNA polymerase II promoter cellular metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process transcription by RNA polymerase II organic substance metabolic process organic substance biosynthetic process transcription initiation at RNA polymerase II promoter cellular metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process DNA-templated transcription initiation organic cyclic compound metabolic process biosynthetic process organic cyclic compound biosynthetic process metabolic process cellular process macromolecule metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular anatomical structure cytoplasm cellular anatomical entity cytosol RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme protein-containing complex nucleus membrane-enclosed lumen nuclear protein-containing complex transcription regulator complex transferase complex intracellular organelle lumen intracellular organelle nucleoplasm catalytic complex intracellular membrane-bounded organelle transferase complex, transferring phosphorus-containing groups nuclear DNA-directed RNA polymerase complex organelle lumen organelle RNA polymerase complex transcription factor TFIID complex DNA-directed RNA polymerase complex intracellular protein-containing complex nuclear lumen transcription factor TFIIE complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR021600	Transcription factor TFIIE alpha subunit, C-terminal	Domain
A	IPR024550	TFIIEalpha/SarR/Rpc3 HTH domain	Domain
A	IPR013083	Zinc finger, RING/FYVE/PHD-type	Homologous Superfamily
A	IPR013137	Zinc finger, TFIIB-type	Domain
A	IPR039997	Transcription factor E	Family
A	IPR017919	Transcription factor TFE/TFIIEalpha HTH domain	Domain
A	IPR002853	Transcription initiation factor IIE subunit alpha, N-terminal	Domain
B	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
B	IPR003166	Transcription factor TFIIE beta subunit, DNA-binding domain	Domain
B	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
B	IPR040501	TFA2, Winged helix domain 2	Domain
B	IPR016656	Transcription initiation factor TFIIE, beta subunit	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P29083	breast cancer malignant mesothelioma hepatocellular carcinoma ovarian cancer lung cancer
B	Pharos: P29084	trichothiodystrophy 6, nonphotosensitive ovarian cancer photosensitive trichothiodystrophy trichothiodystrophy

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.