Annotations: 4ZBJ

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	ASF1-like	8055363	3002057	SCOP2B (2022-06-29)
B	SCOP2 Family	Nucleosome core histones	8070534	4000793	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Core histone-like	8070535	3001387	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	Core histone-like	8042439	3001387	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF04729	e4zbjA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF04729	ECOD (1.6)
B	PF00125	e4zbjB1	A: alpha arrays	X: Histone-like	H: Histone-related	T: Histone	F: PF00125	ECOD (1.6)
C	PF15511	e4zbjC1	A: alpha arrays	X: Histone-like	H: Histone-related	T: Histone	F: PF15511	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.40.1490	Mainly Beta	Sandwich	Immunoglobulin-like	Histone chaperone ASF1-like	CATH (4.3.0)
B	1.10.20.10	Mainly Alpha	Orthogonal Bundle	Histone, subunit A	Histone, subunit A	CATH (4.3.0)
C	1.10.20.10	Mainly Alpha	Orthogonal Bundle	Histone, subunit A	Histone, subunit A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF04729	ASF1 like histone chaperone (ASF1_hist_chap)	ASF1 like histone chaperone	-	Family
B	PF00125	Core histone H2A/H2B/H3/H4 (Histone)	Core histone H2A/H2B/H3/H4	-	Domain
C	PF15511	Centromere kinetochore component CENP-T histone fold (CENP-T_C)	Centromere kinetochore component CENP-T histone fold	CENP-T is a family of vertebral kinetochore proteins that associates directly with CENP-W. The N-terminus of CENP-T proteins interacts directly with the Ndc80 complex in the outer kinetochore. Importantly, the CENP-T-W complex does not directly asso ...	CENP-T is a family of vertebral kinetochore proteins that associates directly with CENP-W. The N-terminus of CENP-T proteins interacts directly with the Ndc80 complex in the outer kinetochore. Importantly, the CENP-T-W complex does not directly associate with CENP-A, but with histone H3 in the centromere region. CENP-T and -W form a hetero-tetramer with CENP-S and -X and bind to a ~100 bp region of nucleosome-free DNA forming a nucleosome-like structure. The DNA-CENP-T-W-S-X complex is likely to be associated with histone H3-containing nucleosomes rather than with CENP-nucleosomes. This domain is the C-terminal histone fold domain of CENP-T, which associates with chromatin [2-3].	Domain
D	PF08729	HPC2 and ubinuclein domain (HUN)	HPC2 and ubinuclein domain	HPC2 (Histone promoter control 2) is required for cell-cycle regulation of histone transcription [1]. It regulates transcription of the histone genes during the S-phase of the cell cycle by repressing transcription at other cell cycle stages. HPC2 m ...	HPC2 (Histone promoter control 2) is required for cell-cycle regulation of histone transcription [1]. It regulates transcription of the histone genes during the S-phase of the cell cycle by repressing transcription at other cell cycle stages. HPC2 mutants display synthetic interactions with FACT complex which allows RNA Pol II to elongate through nucleosomes [2]. Hpc2 is one of the proteins of one of the multi-subunit complexes that mediate replication- independent nucleosome assembly, along with histone chaperone proteins. the Hip4 sequence from SCH. pombe is an integral component of this complex that is required for transcriptional silencing at multiple loci [3]. HPC2, ubinuclein/yemanuclein, and the cell cycle regulator FLJ25778 share a conserved domain that is predicted to bind histone tails [4]. This domain is also referred to as the HRD or Hpc2-related domain.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Histone chaperone ASF1	protein binding binding histone binding enzyme regulator activity enzyme activator activity molecular function activator activity acetyltransferase activator activity molecular function regulator activity	protein-containing complex organization protein-DNA complex assembly protein-DNA complex organization cellular component organization cellular component biogenesis cellular component assembly chromatin organization protein-containing complex assembly chromatin remodeling cellular process cellular component organization or biogenesis nucleosome assembly nucleosome organization cellular response to stimulus protein-containing complex organization protein-DNA complex assembly protein-DNA complex organization cellular component organization cellular component biogenesis cellular component assembly chromatin organization protein-containing complex assembly chromatin remodeling cellular process cellular component organization or biogenesis nucleosome assembly nucleosome organization cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition response to stimulus negative regulation of biological process regulation of cellular response to stress negative regulation of cell cycle regulation of signal transduction intracellular signal transduction cell communication regulation of cell cycle checkpoint negative regulation of cell cycle phase transition signal transduction cell cycle regulation of biological process regulation of cell cycle process DNA integrity checkpoint signaling regulation of response to stimulus negative regulation of DNA damage checkpoint negative regulation of cell cycle checkpoint DNA damage checkpoint signaling signaling negative regulation of signaling negative regulation of intracellular signal transduction positive regulation of biological process cell cycle phase transition negative regulation of cell communication cellular response to stress regulation of intracellular signal transduction response to stress regulation of response to stress negative regulation of cellular process positive regulation of cell cycle process regulation of cell cycle positive regulation of cellular process cell cycle process regulation of signaling negative regulation of response to stimulus biological regulation negative regulation of cell cycle process negative regulation of signal transduction positive regulation of cell cycle cell cycle checkpoint signaling DNA damage response positive regulation of cell cycle phase transition signal transduction in response to DNA damage regulation of cell communication regulation of DNA damage checkpoint subtelomeric heterochromatin formation cellular biosynthetic process telomere organization negative regulation of cellular metabolic process organic substance metabolic process organic substance biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process heterochromatin formation epigenetic regulation of gene expression cellular metabolic process negative regulation of metabolic process chromosome organization regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression organelle organization negative regulation of gene expression constitutive heterochromatin formation biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of gene expression, epigenetic regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of metabolic process DNA replication-dependent chromatin assembly regulation of protein modification process regulation of protein phosphorylation regulation of primary metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process protein metabolic process protein modification process phosphorus metabolic process organonitrogen compound metabolic process protein phosphorylation phosphorylation regulation of protein metabolic process primary metabolic process macromolecule modification regulation of phosphorylation regulation of phosphorus metabolic process RNA metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process transcription by RNA polymerase II regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of transcription by RNA polymerase II nucleobase-containing compound biosynthetic process nucleic acid metabolic process organic cyclic compound metabolic process organic cyclic compound biosynthetic process regulation of DNA-templated transcription silent mating-type cassette heterochromatin formation positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of macromolecule metabolic process regulation of DNA-templated transcription elongation positive regulation of nucleobase-containing compound metabolic process regulation of transcription elongation by RNA polymerase II positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process transcription elongation by RNA polymerase II positive regulation of DNA-templated transcription positive regulation of transcription elongation by RNA polymerase II positive regulation of cellular metabolic process positive regulation of biosynthetic process positive regulation of DNA-templated transcription, elongation DNA-templated transcription elongation positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process cellular component disassembly protein-DNA complex disassembly protein-containing complex disassembly nucleosome disassembly DNA repair DNA metabolic process regulation of DNA repair regulation of DNA metabolic process	protein-containing complex intracellular anatomical structure catalytic complex protein-DNA complex chromatin chromosome transferase complex cellular anatomical entity H3 histone acetyltransferase complex histone acetyltransferase complex acetyltransferase complex intracellular organelle organelle intracellular non-membrane-bounded organelle protein-containing complex intracellular anatomical structure catalytic complex protein-DNA complex chromatin chromosome transferase complex cellular anatomical entity H3 histone acetyltransferase complex histone acetyltransferase complex acetyltransferase complex intracellular organelle organelle intracellular non-membrane-bounded organelle protein acetyltransferase complex intracellular protein-containing complex non-membrane-bounded organelle cytoplasm cytosol chromosome, telomeric region chromosomal region membrane-bounded organelle nucleus intracellular membrane-bounded organelle
B	Histone H3	DNA binding binding organic cyclic compound binding nucleic acid binding protein heterodimerization activity protein binding protein dimerization activity structural molecule activity structural constituent of chromatin	-	protein-containing complex intracellular anatomical structure protein-DNA complex nucleosome chromatin chromosome cellular anatomical entity intracellular organelle organelle intracellular non-membrane-bounded organelle non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle
C	Histone H4	DNA binding binding organic cyclic compound binding nucleic acid binding protein heterodimerization activity protein binding protein dimerization activity structural molecule activity structural constituent of chromatin	-	protein-containing complex intracellular anatomical structure protein-DNA complex nucleosome chromatin chromosome cellular anatomical entity intracellular organelle organelle intracellular non-membrane-bounded organelle non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle
D	Ubinuclein-1	DNA binding binding organic cyclic compound binding nucleic acid binding	protein-containing complex organization protein-DNA complex assembly protein-DNA complex organization cellular component organization cellular component biogenesis cellular component assembly chromatin organization protein-containing complex assembly chromatin remodeling cellular process cellular component organization or biogenesis nucleosome assembly nucleosome organization	cell-cell junction apical junction complex bicellular tight junction tight junction anchoring junction cell junction cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nuclear body cell-cell junction apical junction complex bicellular tight junction tight junction anchoring junction cell junction cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nuclear body intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen centrosome microtubule organizing center cytoskeleton centriolar satellite intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle PML body

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR017282	Histone deposition protein Asf1	Family
A	IPR036747	Histone chaperone ASF1-like superfamily	Homologous Superfamily
A	IPR006818	Histone chaperone ASF1-like	Family
B	IPR009072	Histone-fold	Homologous Superfamily
B	IPR007125	Histone H2A/H2B/H3	Domain
B	IPR000164	Histone H3/CENP-A	Family
C	IPR009072	Histone-fold	Homologous Superfamily
C	IPR001951	Histone H4	Family
C	IPR035425	CENP-T/Histone H4, histone fold	Domain
C	IPR019809	Histone H4, conserved site	Conserved Site
C	IPR004823	TATA box binding protein associated factor (TAF), histone-like fold domain	Domain
D	IPR026947	Ubinuclein middle domain	Domain
D	IPR014840	Hpc2-related domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
D	Pharos: Q9NPG3	hydrops fetalis, idiopathic osteosarcoma prostate carcinoma malignant mesothelioma ovarian cancer psoriasis non-immune hydrops fetalis Pick disease

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
D	ACE	RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 PSI-MOD : N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.