6FJW

Streptococcus thermophilus Cas6

PDB DOI: https://doi.org/10.2210/pdb6FJW/pdb

Classification: HYDROLASE
Organism(s): Streptococcus thermophilus
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2018-01-23 Released: 2019-02-06
Deposition Author(s): Tamulaitiene, G., Mogila, I., Siksnys, V., Tamulaitis, G.
Funding Organization(s): Research council of Lthuania

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.70 Å
R-Value Free: 0.252
R-Value Work: 0.213
R-Value Observed: 0.217

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Streptococcus thermophilus Cas6

Mogila, I., Kazlauskiene, M., Tamulaitiene, G., Siksnys, V., Tamulaitis, G.

To be published.

Macromolecule Content

Total Structure Weight: 28.73 kDa
Atom Count: 1,871
Modelled Residue Count: 239
Deposited Residue Count: 243
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Cas6 protein	A	243	Streptococcus thermophilus	Mutation(s): 0 Gene Names: cas6
UniProt
Find proteins for A0A0A7HF73 (Streptococcus thermophilus) Explore A0A0A7HF73 Go to UniProtKB: A0A0A7HF73
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0A0A7HF73
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
CA Query on CA Download Ideal Coordinates CCD File SDF format, chain B [auth A] MOL2 format, chain B [auth A]	B [auth A]	CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N		Interactions Focus chain B [auth A] Interactions & Density Focus chain B [auth A]

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.70 Å
R-Value Free: 0.252
R-Value Work: 0.213
R-Value Observed: 0.217
Space Group: P 3₁ 1 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 91.469	α = 90
b = 91.469	β = 90
c = 72.419	γ = 120

Software Package:

Software Name	Purpose
PHENIX	refinement
XDS	data reduction
SCALA	data scaling
SHELXDE	phasing
PDB_EXTRACT	data extraction

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2019-02-06

Deposition Author(s):

Tamulaitiene, G.

Funding Organization	Location	Grant Number
Research council of Lthuania	Lithuania	APP-3/2016

Revision History (Full details and data files)

Version 1.0: 2019-02-06
Type: Initial release

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.