5YEK

Crystal structure of BioQ

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.19 Å
  • R-Value Free: 0.247 
  • R-Value Work: 0.212 
  • R-Value Observed: 0.214 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history. 


Literature

Structural insights into operator recognition by BioQ in the Mycobacterium smegmatis biotin synthesis pathway.

Yan, L., Tang, Q., Guan, Z., Pei, K., Zou, T., He, J.

(2018) Biochim Biophys Acta Gen Subj 1862: 1843-1851

  • DOI: https://doi.org/10.1016/j.bbagen.2018.05.015
  • Primary Citation of Related Structures:  
    5YEJ, 5YEK

  • PubMed Abstract: 

    Biotin is an essential cofactor in living organisms. The TetR family transcriptional regulator (TFTR) BioQ is the main regulator of biotin synthesis in Mycobacterium smegmatis. BioQ represses the expression of its target genes by binding to a conserved palindromic DNA sequence (the BioQ operator). However, the mechanism by which BioQ recognizes this DNA element has not yet been fully elucidated.

    • Organizational Affiliation: 

    College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.


Macromolecules
Find similar proteins by: 
(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
TetR family transcriptional regulator
A, B
232Mycolicibacterium smegmatis MC2 155Mutation(s): 0 
Gene Names: MSMEI_3111
UniProt
Find proteins for A0QX69 (Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Explore A0QX69 
Go to UniProtKB:  A0QX69
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupA0QX69
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.19 Å
  • R-Value Free: 0.247 
  • R-Value Work: 0.212 
  • R-Value Observed: 0.214 
  • Space Group: I 1 2 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 37.905α = 90
b = 193.941β = 100.2
c = 54γ = 90
Software Package:
Software NamePurpose
PHENIXrefinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing

Structure Validation

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View more in-depth experimental data
Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2018-09-26
    Type: Initial release
  • Version 1.1: 2018-10-03
    Changes: Data collection, Database references
  • Version 1.2: 2023-11-22
    Changes: Data collection, Database references, Refinement description