1EFA
CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF
- PDB DOI: https://doi.org/10.2210/pdb1EFA/pdb
- NAKB: 1EFA
- Classification: TRANSCRIPTION/DNA
- Organism(s): Escherichia coli
- Expression System: Escherichia coli
- Mutation(s): Yes 
- Deposited: 2000-02-07 Released: 2000-03-06 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.60 Å
- R-Value Free: 0.264 
- R-Value Work: 0.247 
- R-Value Observed: 0.247 
wwPDB Validation   3D Report Full Report
This is version 2.2 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
LAC REPRESSOR | C [auth A], D [auth B], E [auth C] | 333 | Escherichia coli | Mutation(s): 1  | |
UniProt | |||||
Find proteins for P03023 (Escherichia coli (strain K12)) Explore P03023  Go to UniProtKB:  P03023 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | P03023 | ||||
Sequence AnnotationsExpand | |||||
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Find similar nucleic acids by: Sequence | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Length | Organism | Image | |
DNA (5'-D(*GP*AP*AP*T*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*T)-3') | A [auth D], B [auth E] | 21 | N/A | ||
Sequence AnnotationsExpand | |||||
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Small Molecules
Ligands 1 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
NPF Query on NPF | F [auth A], G [auth B], H [auth C] | 2-nitrophenyl beta-D-fucopyranoside C12 H15 N O7 SWRPIVXPHLYETN-BVWHHUJWSA-N |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.60 Å
- R-Value Free: 0.264 
- R-Value Work: 0.247 
- R-Value Observed: 0.247 
- Space Group: H 3 2
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 251.438 | α = 90 |
b = 251.438 | β = 90 |
c = 204.791 | γ = 120 |
Software Name | Purpose |
---|---|
AMoRE | phasing |
CNS | refinement |
DENZO | data reduction |
SCALEPACK | data scaling |
Entry History 
Deposition Data
- Released Date: 2000-03-06  Deposition Author(s): Bell, C.E., Lewis, M.
Revision History (Full details and data files)
- Version 1.0: 2000-03-06
Type: Initial release - Version 1.1: 2008-04-27
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2011-11-16
Changes: Atomic model - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Derived calculations, Structure summary - Version 2.1: 2021-11-03
Changes: Database references, Structure summary - Version 2.2: 2024-02-07
Changes: Data collection